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SCU Quick Guide PTM-ITLAP-004 REV01 Cust Doc ENGLISH

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Safety SubCulture Unit Quick Guide Note: This document is a product use reference guide. Please consult the package insert for the complete Instructions for Use. Insert A) Remove SCU from B) Hold bottle with one C) Hold SCU in place package and position hand. With other hand, with one hand and SCU tip at center of press down on SCU using other hand, pull disinfected bottle flanges to pierce bottle up on white filter cap stopper. top. to remove. Note: The SCU will not sit flush with the stopper. Drop A) Position SCU B) Tilt bottle to C) Replace white filter sampling channel dispense drop. cap when finished Method close to media plate dispensing drops. or slide. Syringe A) Aseptically remove B) Insert male end of C) Upright bottle and the sampling channel. syringe into female luer syringe. Pull up on Method For example, place an of SCU. Invert bottle syringe plunger to alcohol wipe over and syringe and clear any remaining insert and pull up with withdraw required culture in the SCU. finger tips. volume into syringe. D) Replace white filter Remove A) Hold bottle with one cap as described hand and with other

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D) Replace white filter Remove A) Hold bottle with one cap as described hand and with other above in the Drop and hand twist and pull up Method. Dispense Discard on SCU to remove. sample from syringe. SCU Discard into appropriate bio-hazard container. PTM-ITLAP-004 REV01 ITL Corporation t: +(1) 888 411 2851 f: +(1) 703 435 6717 Sharps Safety & Best Practice sales@itlus.com www.itlcorporation.com Copyright 2009 ITL Corporation. All rights reserved.

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Safety SubCulture Unit Tips & Tricks General Guidelines When dispensing to a Tilt bottle to smaller angle Prepare to upright series of slides or other (approx. 30-45 degrees) bottle quickly to cut off media, dispense drops above horizontal for better the drop size and rate. sequentially without control of drop size and uprighting the bottle speed. between drops. Note: Gaseous samples tend to flow more quickly. Resin Media To reduce potential Increase the angle of If resin obstructs the SCU clogging, turn the bottle the bottle to dispense tip inside the bottle, tilt the horizontal allowing the drops. wide end of the bottle resin to settle along the downward to clear the resin side of the bottle prior to from the tip. Allow the resin dispensing drops. to settle along the side of the bottle prior to dispensing additional drops. Charcoal Media To reduce potential Clogging may be Clogging may also be clogging, tilt the bottle cleared by gently cleared by replacing the approx. 30-45 degrees pressing the base of SCU filter cap and gently above horizontal. Do the SCU against the tapping the base of the not invert the bottle, as septum while the bottle bottle on a counter. this may increase is at a slight angle.

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the SCU against the tapping the base of the not invert the bottle, as septum while the bottle bottle on a counter. this may increase is at a slight angle. clogging. An alternative is to use the syringe method. PTM-ITLAP-004 REV01 ITL Corporation t: +(1) 888 411 2851 f: +(1) 703 435 6717 Sharps Safety & Best Practice sales@itlus.com www.itlcorporation.com Copyright 2009 ITL Corporation. All rights reserved.